Blood transfusion is a necessary treatment for many medical conditions, including blood disorders, cancer, surgery, trauma and childbirth. The diagnostics group have several ongoing projects in progress to aid different aspects of the blood transfusion industry. Our goal is to ensure testing is low cost, rapid, easy to use and accurate.
Current projects include
- hand held device to identify blood group, with a legible result
- blood typing mechanisms on paper, including capillary flow modelling
- cellulose gels for a blood typing alternative,
- red cell longevity,
- techniques to enhance pre-transfusion testing
The GLIF (Group Legible Immunohaemetology Format) has been developed by the diagnostics branch of BioPRIA. This product is now available from Haemokinesis. All it takes is a drop of blood, wait for 60 seconds, wash until the background is clear, then simply read the result.
To further develop our understanding of paper diagnostics the fundamental wicking process in paper is being investigated. This information will ultimately be essential in the design of future diagnostics. Different fluids including biologicals are being investigated using high speed cameras, to observe and consequently model their movement through capillary action in paper.
Fig. 1 Positive result
Fig. 2 Negative result
Current blood typing technologies use gel cards. In these cards the testing reagent (antisera) and red cell solutions are allowed to interact, followed by centrifugation. Agglutinated cells remain at the top, while individual cells will spin down to the bottom. BioPRIA has succeeded in producing a suitable alternative with the cellulose gel (see figure) we are now investigating the binding of antibodies to the cellulose, for future diagnostic products.
Red blood cells are not only necessary for transfusion but also as a reagent for laboratory testing. When refrigerated, these cells currently have a storage time of 42 days. In the laboratory setting this means that blood is required to be donates on a monthly basis to keep up supply. BioPRIA is investigating novel methods for long term storage of these cells. In addition, BioPRIA is investigating methods to shorten the time of pre-transfusion testing to ensure that transfusions can occur safely in a more time efficient manner.
Paper‐Based Blood Typing Device That Reports Patient’s Blood Type “in Writing”
M Li, J Tian, M Al‐Tamimi, W Shen
Angewandte Chemie 124 (22), 5593-5597
Effect of protein adsorption on the radial wicking of blood droplets in paper
MJ Hertaeg, RF Tabor, G Garnier – Journal of colloid and interface science, 2018
Dynamics of stain growth from sessile droplets on paper
MJ Hertaeg, RF Tabor, JD Berry, G Garnier – Journal of colloid and interface science. , 2019, Vol.541, p.312-321
Nanocellulose Hydrogel as a Highly Sensitive Matrix for Blood Typing Tests
R Curvello; L Mendoza; H McLiesh; J Manolios; R Tabor; G Garnier, – ACS Applied Bio Materials, submitted 2019
Laser based Immunohaematology
C Manderson; H McLiesh; R Curvello; R Tabor; G Garnier